Sequencing and Data Processing Quote Request * indicates a required field To inquire about working with GeneLab, including quote requests, please fill out the following form. If you need quotes for multiple assay types, submit a separate form for each. For variations within a single assay, such as different sequencing parameters or kit pricing, use the ‘Additional Comments’ field to specify your requirements.TitleName* First Last Email* Enter Email Confirm Email Institution*Study TitleNumber of Samples*Number of Samples is:* An Estimate Exact Source Organism*Source Type*(e.g. leaf, soleus, larvae, whole organism, etc.)Select desired services:* Sample Processing OnlyIncludes the following options: nucleic acid extraction, library preparation and QC, and sequencing. Data Processing OnlyOnly includes data processing. Please fill out all sample processing fields according to how the raw data were generated. For fields to which you do not know the answer, select other and type in “unsure.” Sample and Data ProcessingIncludes the following options: nucleic acid extraction, library preparation and QC, sequencing, and data processing. Other Other desired services*Select assay type and library preparation kit:* Illumina TruSeq Ribo depletion with RiboZero Gold Illumina TruSeq Ribo depletion with RiboZero Plant Illumina TruSeq Ribo depletion with RiboZero Globin Illumina Stranded Total RNA Prep with Ribo-Zero Plus or Ribo-Zero Plus Microbiome Illumina TruSeq mRNA (poly-A selection) NEBNext Ultra II with poly-A (mRNA) QIAgen UPX 3‘ Transcriptome Kit (for bulk RNAseq) Illumina DNA Prep 10X Multiome ATACseq and Gene Expression Not sure Other Specify other assay type and library preparation kit*Select all services requested:* RNA isolation and QC DNA isolation and QC Combined RNA/DNA isolation and QC Library preparation and QC Include internal control, for RNAseq samples (External RNA Controls Consortium – ERCC) Sequencing Raw data (FASTQ and QC) file delivery Processed data and respective QC files (GeneLab Standard Data Processing pipelines) Other Specify other services requested:*Additional comments to services requested(e.g. with and without ERCC, RNA isolation only, DNA/RNA isolation combined, provide a quote for each of the following read depths: 25M reads per sample, 50M reads per sample)If there is a specific nucleotide extraction kit preferred, please type in the name of the kit and provide a link to the kit documentation.Type of sequencing needed (paired-end or single-end)*Review GeneLab Standard RNA Sequencing ParametersSequencing length in basepairs (50 – 250 bp)*Review GeneLab Standard RNA Sequencing ParametersSequencing depth (number of clusters/sample in millions or coverage requirements)*Review GeneLab Standard RNA Sequencing ParametersNASA funding source(e.g. Space Biology, HRP. If anticipated, please indicate)CAPTCHAPhoneThis field is for validation purposes and should be left unchanged.
